Investigation of diagnostic significance of midregional-pro-adrenomedullin (Mr-Pro-Adm), soluble trigger receptor expressed from myeloid cells (sTREM-1) and hematological parameters in cows with pericarditis

Mert Sezer; Yusuf Umut Bati; Hanife Ardahanli; Ersin Tanriverdi; Alican Yilmaz; Enes Akyuz; Ali Haydar Kirmizigul

Mert Sezer assistant professor
Yusuf Umut Bati Kafkas University, Faculty of Veterinary Medicine, Department of Internal Medicine, 36100, Kars, Turkey
Hanife Ardahanli Kafkas University, Faculty of Medicine, Department of Medical Microbiology, 36100, Kars, Turkey
Ersin Tanriverdi Kafkas University, Faculty of Veterinary Medicine, Department of Surgery, 36100, Kars, Turkey
Alican Yilmaz Kafkas University, Faculty of Veterinary Medicine, Department of Surgery, 36100, Kars, Turkey
Enes Akyuz Kafkas University, Faculty of Veterinary Medicine, Department of Internal Medicine, 36100, Kars, Turkey
Ali Haydar Kirmizigul Kafkas University, Faculty of Veterinary Medicine, Department of Internal Medicine, 36100, Kars, Turkey

Keywords: Biomarker; cow; pericarditis; hematology.

Abstract

In this study, it was determined how the levels of Mr-Pro-ADM and sTREM-1 and some biochemical and hematological parameters changed in cows diagnosed with traumatic pericarditis. A total of 60 cows, 40 diseased and 20 healthy, of Simmental breeds between the ages of 1-7 were used in the study. Clinical, radiological, hematological and biochemical examinations were performed on diseased animals brought to the Department of Internal Medicine, Faculty of Veterinary Medicine, Kafkas University, with complaints of loss of appetite, exercise intolerance, rumen atony and recurrent tympani, abdominal tension, constipation, moaning and fatigue. As a result of the examination, it was determined that lactate dehydrogenase (LDH) enzyme activity was statistically significantly higher (p<0.001) and creatine kinase (CK) enzyme activity was significantly higher (p<0.05) in cows diagnosed with pericarditis. In the hematological comparison; Total leukocyte (WBC) and granulocyte (GRA) counts, mean erythrocyte volume (MCV) and mean erythrocyte hemoglobin (MCH) were statistically significantly higher (p<0.001) in cows with pericarditis; Monocyte (Mon) count, mean erythrocyte hemoglobin concentration (MCHC) and platelet count (THR) were found to be significantly higher (p<0.05). However, the erythrocyte count (RBC) was found to be statistically significantly lower (p<0.001). In the comparison of glutaraldehyde coagulation test (GLA) time and vital signs between the diseased animals and the control group, rectal body temperature, pulsation and respiratory rate were statistically significantly higher in diseased animals (p<0.001); It was determined that the glutaraldehyde coagulation test (GLA) duration was significantly lower (p<0.001) in the diseased animals, depending on the severity of inflammation. When inflammatory biomarkers were compared, Mr-Pro-ADM level was significantly higher in the diseased animals (p<0.001); sTREM-1 level was found to be significantly higher (p<0.05). As a result, pericarditis is a disease frequently encountered in cattle farming enterprises and is life-threatening and ends in death. It is formed as a complication of RPT. It is the perforation of the diaphragm by foreign objects such as sharp wires and nails in the reticulum and their penetration into the heart. Clinical, hematological, biochemical tests as well as radiological examinations are very important for diagnosis.